DATE PALM MICROPROPAGATION EBOOK DOWNLOAD

The use of plant tissue culture to supplement propagation by offshoots is necessary. Since the first attempts at date palm propagation by tissue culture (Shroeder. In the present review, we report the results of a number of studies carried out on date palm micropropagation. It also describes different factors. will be useful for rapid, large-scale propagation of 'Khalas' date palm. Key words: Phoenix dactylifera L., tissue culture, plant regeneration.


DATE PALM MICROPROPAGATION EBOOK DOWNLOAD

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DATE PALM MICROPROPAGATION EBOOK DOWNLOAD


Micropropagation of Date Palm: A Review | OMICS International

Epub Mar The date palm Phoenix dactylifera L. Hillawi [ 46 ].

DATE PALM MICROPROPAGATION EBOOK DOWNLOAD

Asil, Hussaini and Zaidi [ date palm micropropagation ]. According to Al-Khateeb, low PGRs concentrations promote the formation of buds while high concentrations induce abnormal growth without bud formation [ 48 ].

DATE PALM MICROPROPAGATION EBOOK DOWNLOAD

Studies on adventitious bud formation from inflorescence explants are very scarce. Loutfi and chlyah indicated that shoot primordia is formed mostly on Greshoff and Doy medium supplemented with 0. In a recent review of date palm micropropagation literature, Abahmane reported that the combination of one auxin and two cytokinins is date palm micropropagation for bud formation on inflorescence explants [ 50 ].

DATE PALM MICROPROPAGATION EBOOK DOWNLOAD

As regard to date palm micropropagation period of offshoot removal, Beauchesne et al. Aissam reported that the explants taken between October and February show the highest buds formation rate [ 51 ], whereas Zaid et al.

Shoot bud multiplication Many factors influence shoot bud multiplication in date palm, especially the basal formulation of the culture medium, the genotype and PGRs. Abahmane mentioned that date palm micropropagation main basal formulation used is MS at full or half-strength, supplemented with PGRs at low concentrations as compared with the bud initiation stage date palm micropropagation 39 ].

For cultivar Khalas, Aslam and Khan used 7. In a previous work on cv. Najda, we found that the best medium for shoot bud multiplication was half-strength MS medium supplemented with 0.

The date palm (Phoenix dactylifera L.) micropropagation using completely mature female flowers.

Mazri recommended MS medium containing 2. Boufeggous, which showed Hillawi, which resulted in an average of Other factors such date palm micropropagation the medium texture [ 5455 ], cultivation in bioreactors [ 56 ], explant size and density [ 57 ] date palm micropropagation carbon source [ 565859 ] were also reported to affect shoot bud multiplication of date palm.

Shoot elongation, rooting date palm micropropagation plantlet acclimatization Shoot elongation and rooting may be achieved either on a medium containing PGRs or on a PGR-free medium.

El Sharabasy et al. The use of liquid medium was also reported to promote shoot elongation [61]. Najda, we compared media with and without PGRs. Our results showed that shoot elongation is fast in media supplemented with PGRs, with high root formation rates. However, shoots cultured on PGR-free media had wider and greener leaves, and exhibited higher survival rates after acclimatization [ 53 ].

This shows that plantlet acclimatization might be influenced by previous culture conditions.

The date palm (Phoenix dactylifera L.) micropropagation using completely mature female flowers.

Along this line, it has been shown that the texture of the elongation-rooting medium influences the survival rate of plantlets after ex vitro transfer.

Indeed, the use date palm micropropagation a liquid medium just before plantlet acclimatization showed lower survival rates as compared to a semi-solid medium [ 5461 ]. On the other date palm micropropagation, increasing the level of sucrose in the elongation-rooting medium increases the survival rate of plantlets during acclimatization [ 59 ].

Other factors such as the nature of the substrate [ 60 ] and the application of gamma aminobutyric acid [ 62 ] were reported to influence plantlet acclimatization.

Problems Encountered in Date Palm Date palm micropropagation Tissue browning Browning of in vitro cultured explants is a frequent problem in date palm micropropagation.